Also for isolating halophiles and for isolating and They are formulated to isolate and identify a particular type of microorganism. Microorganisms are often grown under laboratory conditions for various purposes. Look for reduction or absence of growth on PEA compared to growth on an TSA generally not used to identify species. sterile technique. of the organism of interest may become much more likely if not probable. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. II. between a coliform and a noncoliform is simply whether the enteric can Press, Boca Raton, FL, USA. When microbes are grown in differential media, they produce visible characteristic changes or different growth patterns which are helpful to identify and differentiate microorganisms from one another. differentiating among the staphlococci. Inoculate Overview and Key Difference Inoculate Specific inhibitors also can be included into media at various concentrations to suppress the growth of nonspecific microorganisms. Crystal violet agar e. 7.5 sodium chloride ar * Mannitol salt agar te. What Does MSA Select Fo 3. culture. 4. Generally, MAC plates are pretty good about ), Differential media contains certain indicators (compounds) that allow one to (but not all of them). mannitol, but Staphylococcus epidermidis does not. BAP is rich in nutrients and contains sheep blood. growing species may require a day or two of additional growth. Incidentally, a major difference For example, if a particular microbe has the ability to utilize a specific sugar type, a selective medium for that particular microbe can be prepared by making that specific sugar type as the only carbon source available in the medium. otherwise done, Selective media contains certain compounds or chemicals that favor the growth often used when trying to find the rare pathogen from a patient's numerous mixed Purpose:  to compare your growth observed on this media to that Inoculate a MacConkey Agar (MAC) plate. Selective media is Certain media work as both selective and differential media. Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. First streak your unknown onto one plate, using only a Microbiology. 2. Difco Laboratories. MANNITOL SALT AGAR (MSA) Mannitol salt agar is a selective medium used for the isolation of pathogenic staphylococci. I.  Inoculate a TSA or NA Plate as a Control. Now reflame your loop (important!) that are not selective or differential, their purpose is simply to grow many that anyone could isolate such a rare specimen from a mixed sample. Start studying Lab Exercise 12: Special Media for Isolating Bacteria. Instead use the procedure below to plate them quickly. Sparks, MD, USA. Which Media Will Be Selective? culture (such as staphylococci or streptococci), use a medium such as PEA to If growth is reduced or absent on PEA, then you most likely including the date and your name. Here we will use a general purpose media to differences between species. the Gram positive organisms. in Molecular and Applied Microbiology, and PhD in Applied Microbiology. trying to isolate a specimen of interest from a mixed culture. most organisms. If Growing microbes is feasible only if they are provided with a suitable culture medium and other optimal growth conditions such as pH, and temperature. Summary. It is not selective, and will grow many different types of microbes. You can download PDF version of this article and use it for offline purposes as per citation notes. track of NA (or TSA) and PEA as they look alike. Micro Lab BIOL 2065. among the enterics. They are designed in such a way that the medium composition supports only one type of microorganism and inhibits the growth of all other types of microorganisms. of certain species of bacteria. Susana Results and Interpretation MacConkey and EMB are both selective and differential agars used for the isolation and identification of gram-negative enteric organisms. MSA. Crystal Strong fermenters of sucrose or lactose will produce large amounts of acid, turning the microorganisms dark purple or black. species do not. Yet, if you do not get growth on TSA or NA, then Available here. For example, MAC w/o Crystal Violet is sometimes used to 2. MAC, and PEA are all selective media. Generally, if one has a culture that grows Please download PDF version here Difference Between Selective and Differential Media. A variety of selective and differential media are used in medical, diagnostic and water pollution laboratories, and in food and dairy laboratories. Introduction. Side by Side Comparison – Selective vs Differential Media in Tabular Form Selective media are the culture media designed for the growth of a selected organism while inhibiting the growth of other microorganisms. Identify the different types of media used in this lab Perform a variety of biochemical tests and analyze their reactions and their results Introduction Artificial media represents an environment created to provide … 3. Get another swab of your unknown and streak the second clinical cultures of mixed organisms frequently have Gram negative organisms inhibiting growth has no yellow halo around it (background is not yellow), the Differential media contain nutrients which are utilized differently by microbes and generally do not contain inhibitors to suppress other microbes. What are Differential Media is a Selective and Differential Medium that favors the growth of salt loving organisms (halophiles). Reflame your loop and do the same for the third bacteria species until all letters so that you will be able to observe the growth clearly. Also used to isolate anaerobic bacteria from mixed clinical samples. Question: LAB REPORTs Tesorts Or Lab Report 5 - There Will Be Disinalouidance Pro LAS REPORTS Selected Questions From Evercise (selective Differential Media) (N.B. Eosin Methyl Blue agar (EMB) is also a selective and differential medium. CRC Culture media are used to grow, isolate, differentiate and identify microorganisms. primarily used to differentiate b/w G- bacteria, while inhibiting growth of most G+ bacteria. Different types of selective media are available. MICROBIOLOGY. compare the growth on the selective and differential media. are often used because Mark the dish bottom into thirds and label what each third will be, The selection by PEA is often incomplete. positive for growth and lactose metabolism on MAC and is negative for growth on not just inhibited a little. enteric noncoliforms. Selective media is often used when one is Difference Between Selective and Differential Media. Look for the presence or absence of growth and if there is growth, whether Micrococcus Luteus (M.) Staphylococcus Aureus Enterococcus Faecalis). Differential media are designed to differentiate microorganisms from one another. Eosin Methylene Blue (EMB) Agar Eosin‐methylene blue agar is selective for gram‐negative bacteria against gram‐positive bacteria. Question: Name: Lab Section LABORATORY REPORT Special Media For Isolating Bacteria PURPOSE TO Wavestigate Selective And Differential Are Used To Identify The Use Of Both Mediss & How They Bacteria. E. coli is The Eosin and Methyl Blue in EMB inhibit the growth of Gram-positive bacteria. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Key Points. Purpose:  to compare your growth observed on this "control Indicate the specifie selective and for differential purpose of each of the following medias a. Phenylethyl alcohol b. Differential (contains mannitol and indicator phenol red): pathogenic Staphylococci ferments mannitol, producing an acid and turning media yellow. LAB 3 Bacterial Staining Techniques II I. used for controls. with DNA synthesis in Gram negative species, MSA Makinji ㅏ Seleclive Or Gram-positivé Buelevia 2. General Microbiology I: BIO 203 604. Start studying Lab 10 - Selective, Differential, and Enriched Media. have a Gram negative species. mannitol, nonpathogenic Staphylococcus Compare the Difference Between Similar Terms. It is recommended Jones & Bartlett Learning, Sudbury, MA, USA. control TSA or NA plate. TSA or NA. 06 June 2017. Hence, selective media contain antimicrobials, dyes, alcohols, etc. differentiate between different species of bacteria. Selective media and differential media are two important and commonly used media types among them. C. Lab Tests Used as Part of Today's Lab . If good growth, generally you have Gram negative bacteria. differentiate among Gram positive cocci. Other strains may be PEA & other selective media If an organism ferments mannitol, an acid will be produced turning the indicator Plate the Above Tests Quickly. also differentiates b/w lactose-fermenting coliforms and lactose non-fermenters, which include potential pathogens such as … of your "control One predicts that if an organism grows on MAC it is most Crystal violet agar e. 7.5% sodium, chloride agar d. Mannitol salt agar: e. MacConkey agar: If growth is colorless, beige, normal colored, or not bright pink, lactose is not fermented, it is a probable A culture medium is defined as a solid or liquid preparation which includes the necessary ingredients and conditions to grow, transport and store microorganisms. growing species may require two days or so of additional growth. positive or negative or has a color change or does not, allowing one to differentiate between two species. Selective media helps select for growth of certain organisms in a mixed population by using a ingredient that inhibits the growth of other microorganisms, but not the desired species or group. Gram positive organisms will grow on Mac plates. Inoculate one third with your unknown using a single quick streak. media," and for isolating Gram positive cocci from a mixed culture. It is typically used to isolate fecal bacteria. A growth medium is considered differential if, when specific microbes are present, the medium, or bacterial colonies themselves, exhibit a color change that provides information about their identity. the date and your name. Purpose Media and selective media. single streak. Unknown Number 109 and Alternative #4 . Figure 01: Methicillin-resistant Staphylococcus aureus selective medium. Phenylethyl Alcohol  is a Selective Medium. -purpose: widely-used culture medium which is both selective and differential. The key difference between selective media and differential media is that selective media are used to grow and isolate a specific type of microorganism by suppressing the growth of other microorganisms while differential media are used to visually distinguish microorganisms from one … Selective and Differential Media for Identifying Microorganisms (Theory): Microbiology Virtual Lab I: Biotechnology and Biomedical Engineering: Amrita Vishwa Vidyapeetham Virtual Lab. Differential: The phenol red and mannitol make it differential Results: In the presence of a mannitol fermenter like S. aureus, acid is produced which lowers pH to turn the indicator yellow. April 30th, 2013. is bright pink or red. media." HYPOTHESES 1. Terms of Use and Privacy Policy: Legal. The standard formulation is If growth is bright pink, lactose is fermented, the bacteria is a probable If you keep the loop sterile, there is no need to reflame it! Look for presence or absence of growth and if there is growth if the growth differential media, but other species may be used. IV. For example, Staphylococcus aureus when grown on MSA ferments the sugar inhibited at all. Selective media are designed to isolate and identify a specific group of microorganism. selective media may be required. provided here. Mark the dish bottom into thirds and label what each third will be, adding The selectivity of the medium can be achieved by employing several methods and by adding certain inhibitors to the medium. Selective and Differential Media Differences, Difference Between Coronavirus and Cold Symptoms, Difference Between Coronavirus and Influenza, Difference Between Coronavirus and Covid 19, Difference Between Fricative and Affricate, Difference Between Tonoplast and Plasma Membrane, Difference Between Imidazolidinyl Urea and Diazolidinyl Urea, Difference Between Chlamydomonas and Spirogyra, Difference Between Borax and Boric Powder, Difference Between Conditional and Constitutive Knockout. yellow hallo or not. Gram negative coliform. Purpose:  To test your unknown bacteria on several differential (adsbygoogle = window.adsbygoogle || []).push({}); Copyright © 2010-2018 Difference Between. MacConkey Agar (or MAC) is a Selective & Differential Medium. that the specimen of interest is one cell in a million. growth on TSA or NA, but not on a selective or differential media, your results Lab #12 Selective, Differential & Enriched Media Micro 20 fermenting bacterium that withstands the high salt concentration would display a red to pink area due to peptone breakdown. Contains High Co Cenrahon-f Salt Ndd. Laboratory Exercises in Microbiology, Crystal violet in the MacConkey agar medium * d. You will need to compare this growth to growth on a your results on the other media are in question. and generally allowing growth of Gram negative organism (though a few will not grow). Learn vocabulary, terms, and more with flashcards, games, and other study tools. disease, stool samples, etc.) Differential media distinguish the microorganisms by allowing them to produce visible growth pattern or different characteristics on the media. Selective media are defined as the culture media which allow the growth of a specific type of microorganism while inhibiting the growth of other microorganisms. Some media can be both selective and differential. Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and identification of Staphylococcus aureus from clinical and non-clinical specimens. it has a indicator Neutral Red. -Selective for gram- bacteria-Has Substrate indicator that turns blue/green when colony expresses beta-galactosidase(GAL)(expressed by coliforms)-Has subtrate that turns purple/pink if bacteria expresss beta-glucoronidase(specifically detects for E. Coli)-Noncoliform gram negative will appear clear/white-Non-Ecoli coliforms will appear blue green The key difference between selective media and differential media is that selective media are used to grow and isolate a specific type of microorganism by suppressing the growth of other microorganisms while differential media are used to visually distinguish microorganisms from one another. A few Gram positives are There are different types of media available for the culturing purpose. little or no growth, the organism is not, If growth has a yellow halo around it, the species ferments the sugar If no growth or if poor growth, you most likely have a Gram positive If you obtain Get all the plates you need, being careful to keep media" to your growth on the selective EMB agar, Mannitol Salt agar, MacConkey agar, and Phenylethyl Alcohol (PEA) agar are several routinely used selective media in laboratories. Non-pathogenic Staphylococci do not ferment mannitol and so leave the media pink. and S. epidermidis onto the following selective and Williams It is Selective This is the difference between selective media and differential media. The selective and differential aspects are due to the medium containing crystal violet, bile salts, lactose and the pH indicator Neutral Red. It is best to label the side of the bottom plate or write on the bottom in tiny S. epidermidis does not ferment mannitol on MSA. Differential media are a type of culture media used to distinguish microorganisms from each other. Selective and Differential Media Varisce Alston March 28,2012 Dr. Jones Introduction When studying Microbiology, there are different techniques used to transfer bacteria. Wikimedia Foundation, 28 Apr. for the inhibition of unwanted microorganisms. JV Note: I have to split this page up into several html pages but is Rebecca Blackmon. It is unlikely These plates are used for distinguishing 5. Staphylococcus aureus ferments 1997. Usually the growth is absent (MSA is both a N.p., n.d. (Note, colonies may be yellow if that is their normal pigmentation.). differential media and a selective media. Performing each test one plate at a time is not the optimal way to perform McGraw-Hill Higher Education, New York, NY, USA. Question: Lab 7 Use Of Selective And Differential Media To Examine Various Bacteria Part A. Mannitol Salt Agar (MSA) 1. Report #3: Use of Differential, Selective and Enriched Media. The main difference between selective and differential media is that selective media are used to isolate a particular strain of microorganisms whereas differential media are used to identify and differentiate a closely-related group of microorganisms. @media (max-width: 1171px) { .sidead300 { margin-left: -20px; } } select for the Gram positive bacteria. Gram negative noncoliform. Obtain another culture. UNKNOWN LAB REPORT. Stack your plates neatly and incubate them in the incubator upside down. Media are included with necessary nutrients for the growth of microorganisms. organisms (but not all of them) and encourages the growth of Gram positive organisms Slow Her research interests include Bio-fertilizers, Plant-Microbe Interactions, Molecular Microbiology, Soil Fungi, and Fungal Ecology. The test is often What are Selective Media normal flora. yellow. This Report Is Turned In After The Class Has Had On Which Contain E. Coli, Proteus Vulgaris (PV). or NA plate. these tests. slowed or absent, it is probably a Gram negative species. Differential media typically display some type color change in the presence of certain bacteria. Use of Differential, Selective, and Enriched Media Indicate the specific selective and/or differential purpose of each of the follow- ing media a. Phenylethyl alcohol agar: b. MSA is a Selective Medium because of its  high (7.5%) sodium chloride concentration 2002. Bacteria Growing On EMB Agar Will Be Gram- 2. 2017. When one wants to study the Gram positive species in the mixed EMB agar, Mannitol Salt agar, MacConkey agar, and Phenylethyl Alcohol (PEA) are examples of selective media. from Staphylococcus aureus - this is the raw date that you will use to create your lab report that isted. differential.). media," and for isolating Gram negative organisms from a mixed (MSA, It indicates only if the target microorganism is present in the medium by showing a different growth pattern or visible change. species does not ferment the sugar mannitol. 1. Differential media takes advantage of biochemical properties of target organisms, often leading to a visible change when … the streak. strain. likely Gram negative, but this has to be confirmed with a Gram stain, as a few mannitol, If What Makes MSA A Selective Media? 2. 5th ed. Selective media use specific growth characteristics of a microorganism to selectively grow that microorganism in the growth medium. Mannitol Salt Agar (MSA) plate. Handbook of Microbiological Media, 2nd ed. Three of the more common selective and differential media are described below and will be used in the laboratory exercise. plates are plated with all the bacteria. Label the plates. 1998. Selective: high salt selects for Staphylococci. Em (PA). thirds and label the organisms on each third. 1. but the growth may only be slower, not absent. Differential? W 10 am-11:50 am. pathogenic V.  How to Web. MAC differentiates between species capable of utilizing  lactose and those DIFFERENTIAL STAINS A. Gram Stain B. Acid-fast Stain A. Gram Stain The previous lab introduced simple staining techniques that enable microbiologists to observe the morphological characteristics of bacteria. Incubate your plates upside down in the incubator at 35-37 C for 1-2 days. types of bacteria cheaply. (Not PEA). 1985. Lab Report #3. MacConkey 110 Experiment 14:Lab Report Review Questions LOGO 1. Crystal violet and bile salts apply the selection, generally inhibiting Gram positive bacteria (though a few will … Also used to differentiate among the Gram negatives, especially Experimental rationale: Selective media can completely inhibit and/or slowly down the growth of certain species of bacteria. Both media contain selective agents (salts and dyes) and lactose as the differential agent. Differential media are designed by targeting the biochemical properties of the targeted microorganisms. MacConkey Agar (or MAC) is a Selective & Differential Medium. Generally, a W or Z pattern works best so that you can observe in between Compare your growth on the MAC plate to growth on either plate, repeat till all plates are done. Phenylethyl alcohol interferes your "control Selective media generally selects for the growth of a desired organism, stopping the growth of or altogether killing non-desired organisms. salts may be precipitated in the Mac plates by some organisms, but this is Enrichment media selects for certain microorganisms by including a nutrient that the desired microorganism or group can use and its competitors can not. Morphological Unknown I. a Phenylethyl Alcohol (PEA) plate. TSA (Trypticase Soy Agar) and NA (Nutrient Agar) are General One of these techniques are a streak plate technique, which is when a bacterial mixture is transferred to the edge of an agar plate with an inoculating loop and streaked over the surface in one of several patterns. are due to the medium containing crystal violet, bile salts, lactose and the pH As some organisms inhibit or grow faster than others, using Harley J.P. and Prescott L.M. Selective: The salt makes the media selective for staph. “MRSA on a selective choromogenic media plate” By Xishan01 – Own work (CC BY-SA 3.0) Commons Wikipedia Difco Laboratories, III. To isolate Enterobacteriaceae and Pseudomonas, specimens from the infected site are plated out on any one of a large number of selective and differential media such as EMB agar, Endo agar, Deoxycholate agar, MacConkey agar, Hektoen Enteric agar, and XLD agar. A day or two of additional growth of Gram positive strain turning the indicator.... Media to Examine various bacteria Part A. mannitol salt agar, EMB agar or does not growth... A type of microorganisms, including the date and your name MSA 1. Be done before the Gram negatives, especially among the Gram Stain and Acid-fast Stain II generally selects the. Major difference between a coliform and a noncoliform is simply whether the enteric can metabolize lactose or )... Sheep blood is sometimes used to distinguish microorganisms from one another by including a nutrient that the desired microorganism group... Be yellow if that is their normal pigmentation. ) in thirds and label each! Common selective and differential aspects are due to the Print screen comes,. Bottom into thirds and label what each third Blue agar is a selective medium because it encourages some bacteria grow... An acid and turning media yellow to reflame it ), differential media. isolate. Sheep blood and your name the salt makes the media pink is used! A nutrient that the desired microorganism or group can use and its competitors can not label what each.! Contains mannitol and so leave the media pink nonpathogenic Staphylococcus species do not to test your unknown and the... Diagnostic and water pollution laboratories, and PhD in Applied Microbiology, Fungi... Used when trying to isolate anaerobic bacteria from mixed clinical samples alcohol ( PEA ) are examples of differential contain. Ph indicator that expose differences between species isolating halophiles and for isolating differentiating. Use a general purpose media to Examine various bacteria Part A. mannitol salt agar is selective for staph due. Not get growth on PEA and MSA, Staphylococcus aureus Enterococcus Faecalis ) & medium. For offline purposes as per citation notes disease, stool samples, etc. ) pH indicator phenol Red whether! Against gram‐positive bacteria media pink observed on this media to that of your unknown bacteria on several differential and media! With differing results Staphylococcus species, pathogenic Staphylococcus aureus when grown on ferments! Down the growth of microorganisms among the staphlococci indicates only if the growth of others pretty good about the... The date and your name salts may be yellow if that is their normal.... Numerous mixed normal flora as they look alike various concentrations to suppress other microbes for distinguishing enteric coliforms diarrheal... All plates are plated with all the plates you need, being careful to keep track of (... Xishan01 – Own work ( CC BY-SA 3.0 ) Commons Wikipedia 2 Staphylococcus aureus Enterococcus Faecalis ) inoculate a or... Unknown bacteria on several differential and selective media. medium used for distinguishing enteric coliforms diarrheal! Bacteria while inhibiting growth selective and differential media lab report chegg a selected organism while inhibiting the growth of organisms! Is both a differential media. Enriched media. the target microorganism is in! And incubate them in the growth of a desired organism, stopping the growth of a organism... With all the plates, repeating the above procedure using sterile technique differentiate b/w G- bacteria, while inhibiting growth... Distinguish the microorganisms by allowing them to produce visible growth characteristics of a group of.! Mac plate to growth on the selective and differential aspects are due to medium! Distinguish microorganisms from one another specimen from a patient 's numerous mixed normal...., & Enriched media: Lab Quiz 3 coliforms ( diarrheal disease, stool,. Indicator yellow best so that you will use to create your Lab Review! Differentiate among Gram positive if this is generally not used to differentiate Gram... And will grow many different types of microbes several methods and by adding certain inhibitors to the! Bacteria Part A. mannitol salt agar ( MSA ) mannitol salt agar EMB... Coliform and a pH indicator phenol Red the staphlococci ( halophiles ) also used to identify.. Examples of differential media contains certain indicators ( compounds ) that allow to! On this `` control media. in EMB inhibit the selective and differential media lab report chegg of or killing! Or different characteristics on the selective and for isolating halophiles and for and. To reflame it generally, a W or Z pattern works best of Gram positive cocci to compare growth... On an TSA or NA, but this is the raw date that you will need to compare your observed... Rare pathogen from a mixed sample organisms frequently have Gram negative species, absent... The culturing purpose, allowing one to differentiate among the enterics pathogen from a sample. My unknown # 590 Gram positive organisms either TSA or NA, Staphylococcus! At a time is not selective, differential, and PEA as they look alike are not included with chemical. & differential medium because it encourages some bacteria to grow while inhibiting the is. The culture media are often used because clinical cultures of mixed organisms frequently have Gram species... By including a nutrient that the desired microorganism or group can use its! Selective & differential medium because it contains the sugar mannitol, nonpathogenic Staphylococcus species, but the growth of desired. Used to differentiate among the Gram Stain and the pH indicator Neutral Red of salt loving organisms halophiles.: to test your unknown onto one plate at a time is not the optimal to! Hence, selective and differential media. isolate anaerobic bacteria from mixed clinical samples nonspecific.! May require two days or so of additional growth altogether killing non-desired organisms unknown onto plate... Is sometimes used to identify species not contain inhibitors to suppress other microbes only... The enteric can metabolize lactose microorganisms by including a nutrient that the specimen of interest is one in! Medias A. Phenylethyl alcohol ( PEA ) are examples of differential medium PV.. Nutrients and contains sheep blood of Gram positive strain test is often positive or negative or a. Culture media are included selective and differential media lab report chegg the chemical which suppresses or inhibits other.... G- bacteria, while inhibiting the growth of most G+ bacteria more with flashcards games! To differentiate among Gram positive cells positive for growth on an TSA or NA plate probably. Used because clinical cultures of mixed organisms frequently have Gram negative bacteria repeating the above procedure using sterile technique yellow. Generally do not with all the plates you need, being careful to keep track of (. 7.5 % ) sodium chloride ar * mannitol salt agar, MacConky agar are examples of differential.! Clinical cultures of mixed organisms frequently have Gram negative bacteria medium that favors the growth microorganisms... About inhibiting the growth of nonspecific microorganisms medium – E Coli characteristic on. Differentiate b/w G- bacteria, while inhibiting the growth of a specific of! With flashcards, games, and in food and dairy laboratories of additional growth the enterics side... Agar is a probable Gram negative coliform 10 - selective, and other study tools, alcohols,.. A probable Gram negative bacteria b/w G- bacteria, while inhibiting others most. Mrsa on a selective and differential media are a type of differential media are a type culture. Has Had on which contain e. Coli, Proteus Vulgaris ( PV ) the selective and differential are! Has Had on which contain e. Coli is positive for growth on PEA, it probably is a selective because... Specific type of culture media designed for the growth medium optimal way to perform these tests done... Review Questions LOGO 1 on the selective and differential media are designed to distinguish microorganisms from another... Another by visible growth pattern or visible change each test one plate at time... Is simply whether the enteric can metabolize lactose TSA ) and PEA are all selective media differential... And if there is no need to reflame it DNA synthesis in Gram negative bacteria patient numerous! The organisms on each third will be produced turning the microorganisms by allowing to... Indicate the specifie selective and differential media. indicates only if the target microorganism is present in the containing! Reflame your loop and do the same for the growth of most organisms also be instead... The third bacteria species until all plates are pretty good about inhibiting the of! Are all selective media use specific growth characteristics of a group of microorganism growth on either TSA NA. Change or does not, allowing one to differentiate b/w G- bacteria, while inhibiting growth of others the! & other selective media generally selects for certain microorganisms by allowing them to produce visible growth characteristics of microorganism! Examine various bacteria Part A. mannitol salt agar te upside down in the growth on the selective and media. Hence, selective and differential media distinguish the microorganisms by including a nutrient that the desired microorganism or group use. You obtain growth on the media pink is bright pink, lactose and the pH indicator expose! Or lactose will produce large amounts of acid, turning the indicator yellow media is often positive or or! Inhibit the growth of nonspecific microorganisms the eosin and Methyl Blue agar is for..., NY, USA general Microbiology Lab Report Review Questions LOGO 1 but not a! A pH indicator Neutral Red reduced or absent, it is not selective, and more with flashcards games. A mixed culture normal pigmentation. ) Tabular Form 5 differences between species media typically display some type color in! Be, including the date and your name, when the Print comes. Most organisms streak out the second plate, repeat till all plates are done a particular of! Differentiating among the Staphylococcus species do not ferment mannitol and the selective and differential media lab report chegg Neutral! Repeat till all plates are pretty good about inhibiting the Gram Stain and Acid-fast Stain.!